Omega 3 Testing
Measures percentages of omega-3 fatty acids EPA, DPA and DHA as a potential indicator of various health risks.
DHA Testing
Indicates whether mothers are providing adequate DHA levels to their infants through breastfeeding.
Hair Cortisol Analysis
Provides a complementary method of monitoring stress and cortisol exposure in the body over longer periods of time.
IgG Food Sensitivity
A valuable tool often used to help design elimination diets for patients with several chronic conditions.
Hair Mineral Analysis
Provides the basis for a nutritional balancing program to establish and maintain optimal levels of wellness.

Repletion of Copper-Deficient Rats with Dietary Copper Restores Duodenal Hephaestin Protein and Iron Absorption

Copper (Cu) deficiency in rats reduces the relative concentration of duodenal hephaestin (Hp), reduces iron (Fe) absorption, and causes anemia. An experiment was conducted to determine whether these effects could be reversed by dietary Cu repletion. Five groups of eight weanling male rats each were used. Group 1 was fed a Cu-adequate diet (5.0 mg Cu/kg; CuA) and Group 2 was fed a Cu-deficient diet (0.25 mg Cu/kg; CuD) for 28 days. The rats were fed 1.0 g each of their respective diets labeled with 59Fe (37 kBq/g), and the amount of label retained was measured one week later by whole-body-counting (WBC). Group 3 was fed a CuA diet and Groups 4 and 5 were fed a CuD diet for 28 days. Group 5 was then fed the CuA diet for another week while Groups 3 and 4 continued on their previous regimens. Rats in Groups 3, 4, and 5 were fed 1.0 g of diet labeled with 59Fe, and the amount of label retained was measured by WBC one week later. Rats were killed and duodenal enterocytes isolated for Hp protein analysis, whole blood was analyzed for hematological parameters, and various organs for 59Fe content. CuD rats absorbed less (P,0.05) Fe than CuA rats, the relative amount of duodenal Hp was less (P,0.05) in CuD rats, and the CuD rats developed anemia. After the CuD rats had been repleted with Cu for one week, Fe retention rose to values even higher (P,0.05) than those in CuA rats. After two weeks, the relative amount of duodenal Hp was higher (P,0.05) than normal, and most signs of anemia were reversed. Liver 59Fe was elevated in CuD rats, but was restored to normal upon Cu repletion. These findings suggest a strong association between duodenal Hp abundance and Fe absorption in the CuD rat, and that reduced Fe absorption is an important factor in the cause of anemia.

Phillip G. Reeves and Lana C. S. Demars (Copyright 2005 by the Society for Experimental Biology and Medicine)